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Image Search Results
Journal: The Lancet. Microbe
Article Title: Searching for new therapeutic options for the uncommon pathogen Mycobacterium chimaera : an open drug discovery approach
doi: 10.1016/S2666-5247(21)00326-8
Figure Lengend Snippet: Antimicrobial compound hits identified from screening the Pathogen Box against M chimaera A) Validation of the REMA screening approach for M chimaera , demonstrating an estimated Z factor of 0·87. 50 times rifampicin mean MIC (2·5 μg/mL; red diamond) was compared to 2% dimethyl sulfoxide drug-free media (yellow triangle) in the REMA assay. Data points from three independent experiments were pooled. (B) The 400 Pathogen Box compounds screened against M chimaera, M tuberculosis , and M abscessus at 20 μM with cell survival quantified by REMA. Any compound that inhibited growth by 70% or more were classed as a hit (green bar). Compounds have been classified by MMV as A for cryptosporidiosis; B for kinetoplastids; C for malaria; D for reference compounds; E for schistosomiasis; and F for tuberculosis. (C) Venn diagram describing the overlap in antimicrobial compound hits against M chimaera, M abscessus , and M tuberculosis . The compounds are detailed as a drug name or by the MMV identifier. M chimaera = Mycobacterium chimaera . M tuberculosis = Mycobacterium tuberculosis . M abscessus = Mycobacterium abscessus . MMV=Medicines for Malaria Venture. MIC=minimum inhibitory concentration. RFU=relative fluorescence units. REMA=resazurin microtitre cell viability assay.
Article Snippet: We validated the
Techniques: Biomarker Discovery, Concentration Assay, Fluorescence, Viability Assay